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KMID : 0359320030430010095
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Korean Journal of Veterinary Research
2003 Volume.43 No. 1 p.95 ~ p.102
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Epidemiological Investigation of Staphylococcus aureus Isolated from Bovine Mastitis Based on the Polymorphism of Coagulase Gene
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문진산/Moon, Jin-San
이애리/임숙경/주이석/강현미/김종만/김말남/Lee, Ae-Ri/Lym, Suk-Kyung/Joo, Yi-Seok/Kang, Hyun-Mi/Kim, Jong-Man/Kim, Mal-Nam
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Abstract
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Because Staphylococcus aureus (S. aureus) hs variable number of short sequence repeat region in coagulase gene, it has been used to investigate the relatedness of S. aureus isolates. In this study, we isolated S. aureus strains from 20 dairy farms with bovine mastitis from September 2000 to August 2001. PCR-RFLP analysis of coagulase gene revealed 10 different patterns. Most of the S. aureus isolates showed only one coagulase gene RFLP pattern per farm. However, there were several S. aureus clones spreading between dairy farms. All the farms showed poor management conditions of milking machine and milker, indicating that managements for mastitis control program include use of poor milking matching, premilking sanitation, and segregation in the S. aureus infection herd. Our data suggest that PCR-RFLP analysis of coagulase gene mignt be applicable for the epidemiological investigations of S. sureus isolated from bovine mastitis cows.
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